Journal: Biochemistry

Article Title: Analysis of RNA methylation by phylogenetically diverse Cfr radical SAM enzymes reveals an iron-binding accessory domain in a clostridial enzyme

doi: 10.1021/acs.biochem.9b00197

Figure Lengend Snippet: (A) SSN of IPR025957 constructed with an alignment score of 31. Nodes are arranged in an organic layout and each represents a single sequence. Nodes are shaded by host organism and colored by co-occurring gene type. Clusters without numbering represent groups that do not fall into one of the three major gene type categories. Additional clusters numbering two or less are not shown. (B) Representative gene clusters from each major category as determined by GNN analysis (±10 ORFs, ≥5% co-occurrence). Host organism and cluster number are displayed (left). KTR proteins are marked by a “*” symbol. (C) Breakdown by organism type in each of the first three SSN clusters. Clostridia = blue, Bacilli = purple, and other = green. Pathogenic organisms are highlighted with dark shading and non-pathogens are shown in light shading.

Article Snippet: A BLAST search of the corresponding amino acid sequence shows similarity to an InterPro family (IPR025957) 1 designated “cysteine-rich KTR” with more than 1400 members identified as of May 2019.

Techniques: Construct, Sequencing